human mirna microarray v2.0  (Agilent technologies)

Journal: Molecular Neurobiology

Article Title: MicroRNA Expression Signatures Determine Prognosis and Survival in Glioblastoma Multiforme—a Systematic Overview

doi: 10.1007/s12035-014-8668-y

Figure Lengend Snippet: The biogenesis of miRNA requires RNA polymerase II/III for the transcription of pri-miRNA. The pri-miRNA product is then cleaved by the Drosha-DGCR8 complex into pre-miRNA. The pre-miRNA is exported to the cytoplasm by Exportin-5 in the presence of Ran-GTP co-factor. Once in the cytoplasm, the pre-miRNA is cleaved by the Dicer-TRBP complex into a miRNA duplex, which is unwound into two products: a guide strand bound to Ago2, which is incorporated into the RISC, and a passenger strand, which is degraded. Finally, the miRNA binds to its target mRNAs resulting in mRNA target cleavage, translational repression, or mRNA decay. A more novel fate of the miRNAs is the selective secretion via microvesicles or exosomes. Ran = Ras-related nuclear protein; GTP = guanosine-5′-triphosphate; TRBP = TAR (HIV-1) RNA binding protein; Ago2 = Argonaute protein 2; RISC = RNA-induced silencing complex

Article Snippet: However, four studies used the Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ), which uses the Illumina Human v2.0 miRNA Expression BeadChip microarray platform [ , – ].

Techniques: RNA Binding Assay

Journal: Molecular Neurobiology

Article Title: MicroRNA Expression Signatures Determine Prognosis and Survival in Glioblastoma Multiforme—a Systematic Overview

doi: 10.1007/s12035-014-8668-y

Figure Lengend Snippet: Studies performed on dataset obtained from public databases

Article Snippet: However, four studies used the Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ), which uses the Illumina Human v2.0 miRNA Expression BeadChip microarray platform [ , – ].

Techniques: Biomarker Discovery, In Vitro, Cell Culture, Activity Assay, Standard Deviation, Expressing, Software, Mutagenesis, Amplification

Journal: Molecular Neurobiology

Article Title: MicroRNA Expression Signatures Determine Prognosis and Survival in Glioblastoma Multiforme—a Systematic Overview

doi: 10.1007/s12035-014-8668-y

Figure Lengend Snippet: Studies performed on independent tissue cohorts

Article Snippet: However, four studies used the Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ), which uses the Illumina Human v2.0 miRNA Expression BeadChip microarray platform [ , – ].

Techniques: Control, Biomarker Discovery, In Situ Hybridization, In Vitro, In Situ, Methylation, Expressing, Invasion Assay, Transfection, Amplification, Migration, Luciferase, Transwell Assay, Wound Healing Assay

Journal: Molecular Neurobiology

Article Title: MicroRNA Expression Signatures Determine Prognosis and Survival in Glioblastoma Multiforme—a Systematic Overview

doi: 10.1007/s12035-014-8668-y

Figure Lengend Snippet: miRNAs reported to be protective or risk-associated

Article Snippet: However, four studies used the Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ), which uses the Illumina Human v2.0 miRNA Expression BeadChip microarray platform [ , – ].

Techniques: Expressing

Journal: Molecular Neurobiology

Article Title: MicroRNA Expression Signatures Determine Prognosis and Survival in Glioblastoma Multiforme—a Systematic Overview

doi: 10.1007/s12035-014-8668-y

Figure Lengend Snippet: miRNA signatures correlating with survival in GBM

Article Snippet: However, four studies used the Chinese Glioma Genome Atlas (CGGA) ( http://www.cgga.org.cn ), which uses the Illumina Human v2.0 miRNA Expression BeadChip microarray platform [ , – ].

Techniques: